Incubation:Article Title: Differential frontostriatal ΔFosB dynamics and behavioral flexibility in autism like VPA-exposed mice.
Article Snippet: Autism spectrum disorder (ASD) involves social impairments, repetitive behaviors, and behavioral inflexibility, defined as an inability to adapt behavior to changing contingencies, and is linked to frontostriatal dysfunction.. How behavioral training modulates these circuits is unclear, but ΔFosB, a marker of sustained neural activity, may be useful to establish their relationship.. The aim was to evaluate frontostriatal ΔFosB expression following prenatal exposure to valproic acid (VPA) and to determine how experience in the midsession reversal (MSR) task modulates posterior ΔFosB expression.
Article Title: Evaluation of Modified Chitosan Nanomicelles with Carboplatin for Effective Treatment Against Ovarian Cancer Cell Types in Murine Model
Article Snippet: Sections of tumour (4 μm thick) were hydrated, and heat epitope retrieval (HIER) was performed in microwave oven in citrate buffer pH=6 (Dako Retrieval Solution, Dako, Agilent, Santa Clara, CA, USA). .. After cooling to room temperature (RT), the activity of endogenous peroxidase was blocked with BLOXALL Endogenous Enzyme Blocking Solution (ImmPRESS HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA) washed twice with PBS and further incubated with 2.5% Normal Horse Serum (ImmPRESS HRP Universal PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA). .. Further, slides were incubated with primary monoclonal antibodies: mouse anti- Ki-67 M7240 MIB-1 (Dako, Agilent, Santa Clara, CA, USA) 1h in RT.
Avidin-Biotin Assay:Article Title: Differential frontostriatal ΔFosB dynamics and behavioral flexibility in autism like VPA-exposed mice.
Article Snippet: Autism spectrum disorder (ASD) involves social impairments, repetitive behaviors, and behavioral inflexibility, defined as an inability to adapt behavior to changing contingencies, and is linked to frontostriatal dysfunction.. How behavioral training modulates these circuits is unclear, but ΔFosB, a marker of sustained neural activity, may be useful to establish their relationship.. The aim was to evaluate frontostriatal ΔFosB expression following prenatal exposure to valproic acid (VPA) and to determine how experience in the midsession reversal (MSR) task modulates posterior ΔFosB expression.
Cell-Signaling:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Tissue:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Recombinant:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Stripping:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Gas Chromatography:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Genotyping:Article Title: An African ancestry-enriched H1 sub-haplotype challenges MAPT haplotype assignment and reveals distinct tau regulatory programs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 Abcam Cat# ab5392; RRID: AB_2138153 S100b Sigma Aldrich Cat# s2532; RRID: AB_477499 GREM1 ABclonal Cat# A11595; RRID: AB_2758622 RD3 Millipore Cat# 05-803; RRID: AB_310013 RD4 Millipore Cat# 05-804; RRID: AB_11211556 Total Tau Sigma Aldrich Cat# mab2241; AB_1977340 GADD45G OriGene Technologies Cat# TA505437S GAPDH Abcam Cat# ab181602; RRID: AB_2630358 βIII-tubulin Cell Signaling Cat# 5568; RRID: AB_10694505 goat anti chicken-488 ThermoFisher Scientific Cat# a11039 donkey anti mouse 647 ThermoFisher Scientific Cat# a31571 HRP Goat Anti-Rabbit Vector laboratories Cat# PI-1000 HRP Horse Anti-Mouse Vector laboratories Cat# PI-2000 LRRC37A Thermofisher Scientific Cat# pa5-60086; RRID:AB_2643515 LRRC37A Sigm Aldrich Cat# HPA042121; RRID:AB_2677858 OmniMap anti-rabbit HRP Roche Cat# 05269679001 SMI-312 BioLegend Cat# 837904; RRID:AB_2566782 OmniMap anti-mouse HRP Roche Cat# 05269652001 Biological samples Human postmortem middle frontal gyrus (MFG) and caudate nucleus brain tissue See Tables S7 N/A Chemicals, peptides, and recombinant proteins M-PER ThermoFisher Scientific Cat# 78503 protease/phosphatase cocktail ThermoFisher Scientific Cat# 5872S NuPAGE Bis-Tris 4–12 % Thermo Fisher Scientific Cat# NW04122BOX DAPI Thermo Fisher Scientific Cat# D1306 WesternBright ECL HRP substrate Advansta Cat# K-12045 SuperSignal West Pico PLUS Chemiluminescent Substrate ThermoFisher Scientific Cat# 34580 SuperSignal West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat# 34094 Restore PLUS stripping buffer ThermoFisher Scientific Cat# PI46430 ProLong Gold Antifade Mountant ThermoFisher Scientific Cat# P36935 Invitrogen Novex 10X Bolt Sample Reducing Agent ThermoFisher Scientific Cat# B0009 LDS sample buffer ThermoFisher Scientific Cat# B0007 MES buffer ThermoFisher Scientific Cat# 41320040-2 SyberGreen Mastermix ThermoFisher Scientific Cat# 4309155 TaKaRa LA Taq DNA Polymerase with GC Buffer Clontech Cat# RR02AG Taqman genotyping mastermix Life Technologies Cat# 4371353 doxycycline Sigma Cat# D9891 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 115, 1–17.e1–e12, March 3, 2027 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Global Diversity Array v1.0 BeadChip Illumina https://www.illumina.com/products/by-type/ microarray-kits/infinium-global-diversity.html RNAeasy kit Qiagen Cat# 74104 GSH/GSSG Glo assay Promega Cat# V6611 RNA 6000 Pico Kit Agilent Technologies Cat# 5067-1513 SuperScript IV First-Strand Synthesis System ThermoFisher Scientific Cat# 18091200 Qubit RNA HS Assay Kit ThermoFisher Scientific Cat# Q32855 Qubit dsDNA HS Assay Kit ThermoFisher Cat# Q32853 Bioanalyzer DNA 12000 Kit Agilent Cat# 5067-1508 Quick-RNA Miniprep Kit Zymo Research Cat# R1054 SensiFAST cDNA Synthesis Kit Bioline (Meridian Bioscience) Cat# BIO-65053 SensiFAST SYBR Lo-ROX 2× Master Mix Bioline (Meridian Bioscience) Cat# QP100016 TDE1 Enzyme and Buffer kit Illumina Cat# 20034197 DNA Clean & Concentration Kit Zymo Cat# D4013 Ampure XP beads Beckman Coulter Cat# A63880 KANSL1 TAqman Probe kit Thermo Fisher Cat# Hs03971091_cn-FAM Rs8070723 Taqman genotyping kit Applied Biosystems Cat# C_29297996_10 Rs1052553 Taqman genotyping kit Applied Biosystems Cat# C_7563736_10 TaqMan Copy Number Reference Assay RNaseP; VIC Fluorophore Thermo Fisher Cat# 4401631 PROBE FOR FISH Empire Genomics Cat# WI2-1321L7-red PROBE FOR FISH Empire Genomics Cat# WI2-3237D21-green Deposited data Bulk RNA-seq neurons This paper GEO:GSE335291 Bulk RNA-seq astrocytes This paper GEO:GSE335293 Bulk RNA-seq brains This paper GEO:GSE335405 MAPT Iso-Seq This Paper GEO: GSE328854 ATAC-seq This Paper Available upon request Mass spectrometry proteomics This paper PRIDE: PXD064250 SNP array data This paper Available upon request Long-read sequencing data This paper Available upon request Alzheimer Disease association ADSP https://adsp.niagads.org/data/ Reference panel 100GP, HGDP GnomAD https://storage.googleapis.com/gcp-public- data–gnomad/release/3.1.2/vcf/genomes/ gnomad.genomes.v3.1.2.hgdp_1kg_subset_ sample_meta.tsv.bgz Experimental models: Cell lines Human iPSC lines See Tables S3 and S5 N/A Lymphoblastoid cell lines (LCLs) See Table S3 N/A WTC11 NGN2 cell line Wang et al.55 N/A Oligonucleotides ISO-seq MAPT primers Bowles et al.56 N/A Actin primers Tcw et al.57 N/A Total tau primers Ezerskiy et al.58 N/A GADD45G primers Zhang et al.59 N/A 3R primers Bowles et al.56 N/A 4R primers Bowles et al.56 N/A GAPDH primers This paper See Table S21 for sequence (Continued on next page) ll OPEN ACCESSArticle Neuron 115, 1–17.e1–e12, March 3, 2027 e2
Activity Assay:Article Title: Evaluation of Modified Chitosan Nanomicelles with Carboplatin for Effective Treatment Against Ovarian Cancer Cell Types in Murine Model
Article Snippet: Sections of tumour (4 μm thick) were hydrated, and heat epitope retrieval (HIER) was performed in microwave oven in citrate buffer pH=6 (Dako Retrieval Solution, Dako, Agilent, Santa Clara, CA, USA). .. After cooling to room temperature (RT), the activity of endogenous peroxidase was blocked with BLOXALL Endogenous Enzyme Blocking Solution (ImmPRESS HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA) washed twice with PBS and further incubated with 2.5% Normal Horse Serum (ImmPRESS HRP Universal PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA). .. Further, slides were incubated with primary monoclonal antibodies: mouse anti- Ki-67 M7240 MIB-1 (Dako, Agilent, Santa Clara, CA, USA) 1h in RT.
Article Title: Fourth-generation chimeric antigen receptor T cells targeting mucin 1 for enhanced anti-tumor activity against breast cancer
Article Snippet: .. Endogenous peroxidase activity was blocked with BLOXALL Endogenous HRP/AP Blocking Solution (MP-7602, AB_2336532, Vector Laboratories, CA, USA), followed by 2.5% normal horse serum to block non-specific binding. .. The tissues were then incubated overnight with anti-MUC1 antibody, HMFG2 (MABC1613, AB_3676087, Merck Millipore, Dallas, MA, USA).
Blocking Assay:Article Title: Evaluation of Modified Chitosan Nanomicelles with Carboplatin for Effective Treatment Against Ovarian Cancer Cell Types in Murine Model
Article Snippet: Sections of tumour (4 μm thick) were hydrated, and heat epitope retrieval (HIER) was performed in microwave oven in citrate buffer pH=6 (Dako Retrieval Solution, Dako, Agilent, Santa Clara, CA, USA). .. After cooling to room temperature (RT), the activity of endogenous peroxidase was blocked with BLOXALL Endogenous Enzyme Blocking Solution (ImmPRESS HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA) washed twice with PBS and further incubated with 2.5% Normal Horse Serum (ImmPRESS HRP Universal PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA). .. Further, slides were incubated with primary monoclonal antibodies: mouse anti- Ki-67 M7240 MIB-1 (Dako, Agilent, Santa Clara, CA, USA) 1h in RT.
Article Title: Fourth-generation chimeric antigen receptor T cells targeting mucin 1 for enhanced anti-tumor activity against breast cancer
Article Snippet: .. Endogenous peroxidase activity was blocked with BLOXALL Endogenous HRP/AP Blocking Solution (MP-7602, AB_2336532, Vector Laboratories, CA, USA), followed by 2.5% normal horse serum to block non-specific binding. .. The tissues were then incubated overnight with anti-MUC1 antibody, HMFG2 (MABC1613, AB_3676087, Merck Millipore, Dallas, MA, USA).
Polymer:Article Title: Evaluation of Modified Chitosan Nanomicelles with Carboplatin for Effective Treatment Against Ovarian Cancer Cell Types in Murine Model
Article Snippet: Sections of tumour (4 μm thick) were hydrated, and heat epitope retrieval (HIER) was performed in microwave oven in citrate buffer pH=6 (Dako Retrieval Solution, Dako, Agilent, Santa Clara, CA, USA). .. After cooling to room temperature (RT), the activity of endogenous peroxidase was blocked with BLOXALL Endogenous Enzyme Blocking Solution (ImmPRESS HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA) washed twice with PBS and further incubated with 2.5% Normal Horse Serum (ImmPRESS HRP Universal PLUS Polymer Kit, Peroxidase, Vector Laboratories, USA). .. Further, slides were incubated with primary monoclonal antibodies: mouse anti- Ki-67 M7240 MIB-1 (Dako, Agilent, Santa Clara, CA, USA) 1h in RT.
other:
Saline:Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
Article Snippet: .. Sections were then washed thoroughly in Tris-buffered saline (TBS) before applying horse radish peroxidase-conjugated ImmPRESS anti-mouse IgG (Vector Laboratories, MP-7402) or anti-rabbit IgG (Vector Laboratories, MP-7401) secondary antibodies for 30 min at RT. .. Antibody detection was visualised by incubating the sections for 10 min at RT with 3, 3′-diaminobenzidine (DAB; ImmPACT Vector Laboratories, SK-4105).
Transcranial Magnetic Stimulation:Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
Article Snippet: .. Sections were then washed thoroughly in Tris-buffered saline (TBS) before applying horse radish peroxidase-conjugated ImmPRESS anti-mouse IgG (Vector Laboratories, MP-7402) or anti-rabbit IgG (Vector Laboratories, MP-7401) secondary antibodies for 30 min at RT. .. Antibody detection was visualised by incubating the sections for 10 min at RT with 3, 3′-diaminobenzidine (DAB; ImmPACT Vector Laboratories, SK-4105).
Nonspecific Binding:Article Title: Fourth-generation chimeric antigen receptor T cells targeting mucin 1 for enhanced anti-tumor activity against breast cancer
Article Snippet: .. Endogenous peroxidase activity was blocked with BLOXALL Endogenous HRP/AP Blocking Solution (MP-7602, AB_2336532, Vector Laboratories, CA, USA), followed by 2.5% normal horse serum to block non-specific binding. .. The tissues were then incubated overnight with anti-MUC1 antibody, HMFG2 (MABC1613, AB_3676087, Merck Millipore, Dallas, MA, USA).
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